DNA

Part:BBa_K3280005

Designed by: Amanda Araujo Silva ; Raquel Gonz�lez Sterman ; Luana Corsi Antonio ; Juliana Naomi Yamauti Costa ; Lucas Grizotto Ferreira ; Nayla Naomi Kusimoto Takeuti; Paulina Rossi Ferreira   Group: iGEM19_USP_SaoCarlos-Brazil   (2019-10-16)


Terminator tpr + MerR+ bidirectional Promotor + RBS (Mer operon) segment of BBa_K1355001, a biobrick designed to be the central piece of different genetic constructions related to mercury, which is composed by a bidirectional promoter, a operon expression regulator (MerR), a carrier protein (MerP) and a mercury transport protein (MerT). BBa_k3280005 has only a terminator, a RBS, MerR domain and a bidirectional promoter that has a dual function: in reverse, transcription of the MerR regulator protein and in forward, it is opened for insertion. 

In the absence of mercury MerR represses transcription by binding tightly to the mer operator region; when mercury is present the dimeric complex binds a single ion and becomes a potent transcriptional activator, while remaining bound to the mer site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None